antimycin a  (MedChemExpress)

Journal: bioRxiv

Article Title: TGS1 mediates mRNA 5’-cap trimethylation to promote oxidative phosphorylation in acute myeloid leukaemia

doi: 10.64898/2026.03.18.712389

Figure Lengend Snippet: a. Basal respiration of shCTRL, sh1TGS1 or sh2TGS1 OCI-AML3 cells following 6 days of DOX treatment was measured using the Resipher instrument at the indicated times (hours). b. High-resolution respirometry analyses of shCTRL or sh1TGS1 OCI-AML3 cells following 6 days of DOX treatment, detected by Oroboros. Basal respiration of cells was measured before oligomycin (ATP synthase inhibitor) treatment; maximal respiration of cells was measured in the presence of the uncoupler FCCP. The background was set after treatment with rotenone and antimycin A (AA). c. Basal respiration of shCTRL, shCTRL + Sinefungin 50µM, sh1TGS1, sh1TGS1 + TGS1 WT or sh1TGS1 + TGS1 WT + Sinefungin 50µM OCI-AML3 cells following 6 days of DOX treatment was measured using the Resipher instrument. d. Redox status of Coenzyme Q10 (CoQ10) measured by LC-MC in shCTRL or sh1TGS1 OCI-AML3 cells at the indicated days of DOX treatment (reduced CoQ10 over the sum of reduced and oxidised CoQ10). e. Bioenergetic ratio of shCTRL or sh1TGS1 OCI-AML3 cells at indicated days of DOX treatment estimated by measuring the ratio of ATP/ADP by LC-MS. Values in a , b , c , d and e are mean ± s.d. of n ≥ 3 independent experiments. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001, two-way ANOVA test with Šidák’s ( b , d and e ) or Tuckey’s ( a and c ) multiple comparisons.

Article Snippet: Lastly, 1 μM antimycin A (Cell Signaling, 33357S) and 1 μM rotenone (Tocris 3616/50) were added to inhibit complex III and complex I, respectively.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: Nature Communications

Article Title: VAMP7-dependent late endosomal secretion of ER and mitochondrial proteins impacts the tumor microenvironment and macrophage engagement

doi: 10.1038/s41467-026-69900-4

Figure Lengend Snippet: A – H WT, VAMP7KO, and ATG5KO NRK cells were either untreated (UT) or treated with Antimycin A (AA, 2 µM) or Thapsigargin (Thap, 100 nM) and serum starved overnight. The next day, the secreted media was processed, and total protein was precipitated using acetone. The total secreted fraction and cell lysates were collected and analyzed and the ratio of secreted RTN3A over total RTN3A ( A , B , G , H ) or secreted VDAC over total VDAC ( C , D , E , F ) was plotted. Two-tailed Welch’s t-test was used to determine the statistical significance. Data is derived from three independent experiments and is presented as the mean ± SEM. * p -val < 0.05, ** p -val < 0.01, ns non-significant. I , J WT, VAMP7KO and ATG5KO NRK cells were either left untreated or treated with Thapsigargin (Thap, 1 µM) for 60 minutes, fixed and stained with RTN3 and CD63. Scale bar = 10 µm. Pearson’s Correlation Coefficient (PCC) was measured using ImageJ for the co-occurrence of RTN3 and CD63. One-way ANOVA with Bonferroni’s post test was used to determine the statistical significance. Data is derived from 40 cells from three independent experiments and is presented as the mean ± SEM. **** p -val < 0.0001, ns non-significant. K , L WT, VAMP7KO and ATG5KO NRK cells were either left untreated or treated with Antimycin (AA, 25 µM) for 45 min and stained with Mitotracker and CD63. Scale bar = 10 µm. PCC was measured using ImageJ for the co-occurrence of Mitotracker and CD63. One-way ANOVA with Bonferroni’s post hoc test was used to determine the statistical significance. Data is derived from 40 cells from three independent experiments and is presented as the mean ± SEM. **** p -val < 0.0001, ns non-significant.

Article Snippet: The chemicals and reagents used in the study were: MitoTrackerTM Red CMXRos (ThermoFisher, M7512), Mitotracker Deep Red TM (ThermoFisher, M22426 ), Mitotracker Green TM (ThermoFisher, M7514), ER Tracker Red (ThermoFisher, E34250), Phalloidin Alexa647 (ThermoFisher, A22287), Bafilomycin A1 (Cell Signaling, 54645), Antimycin A (Cell Signaling, 33357), Thapsigargin (Cell Signaling, 12758), Torin1 (Invivogen, Inh-tor1), Fluorescein isothiocyanate–dextran (dextran-FITC) (Sigma-Aldrich, FD10S-100MG), Dextran Alexa FluorTM 647 (ThermoFisher, D22914 ).

Techniques: Two Tailed Test, Derivative Assay, Staining